SOLVED: [15 marks] Total ascorbic acid can be analyzed by spectrophotometric method based on the oxidation of ascorbic acid to dehydroascorbic acid by bromine water in the presence of acetic acid. After coupling with 2,4-dinitrophenylhydrazine (DNPH), a red complex that absorbs at 521 nm is produced. The following data were obtained for a series of ascorbic acid standards:[ascorbic acid] (ppm) No. of replicate Average Standard deviation absorbance at 521 nm0.0 25
SOLVED: [15 marks] Total ascorbic acid can be analyzed by spectrop…
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