Fluorescent protein chromophores are the light‐emitting centres intrinsic to proteins such as green fluorescent protein (GFP) and its variants. These chromophores emerge through post‐translational ...
Discovery of green fluorescent protein / Osamu Shimomura -- Photons for reporting molecular events: green fluorescent protein and four luciferase systems / J. Woodland Hastings and James G. Morin -- ...
Fluorescent proteins have revolutionised biological imaging by offering genetically encodable markers that emit visible light upon excitation. Central to their function is the chromophore, an ...
Modern bioassays tend to fall under three common modalities: colorimetric, luminometric or fluorometric. Colorimetric assays record the amount of light absorbed by a reporter compound. This absorbance ...
It has been over 60 years since Osamu Shimomura et al. discovered Green Fluorescent Protein (GFP) 1. Since then, the color palette for fluorescent proteins has been extended to span blue through to ...